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1.
Int J Nurs Educ Scholarsh ; 21(1)2024 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-38661172

RESUMO

INTRODUCTION: The purpose of this review was to determine the current state of the literature for notetaking among nursing students and identify implications for future nursing education and research. Notetaking is frequently used by college students and is a proven learning strategy. There has been less research for notetaking among nursing students than students in other disciplines. CONTENT: This narrative review was completed using the method described by Ferrari. Seven electronic databases were searched from 2018 to 2023 using the terms notetaking, students, and nursing. SUMMARY: Seven of 65 articles were included in this review. Upon analysis, three categories of research findings were identified: learning style, notetaking skill, and notetaking and technology. OUTLOOK: Nursing faculty should be aware of learning styles, notetaking strategies, and notetaking skill when designing assignments and course content for students. Future research should be done on this population to strengthen the findings of current literature.


Assuntos
Estudantes de Enfermagem , Humanos , Estudantes de Enfermagem/psicologia , Bacharelado em Enfermagem/métodos , Aprendizagem
2.
BMC Genomics ; 25(1): 233, 2024 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-38438840

RESUMO

BACKGROUND: Patagonian toothfish (Dissostichus eleginoides) is an economically and ecologically important fish species in the family Nototheniidae. Juveniles occupy progressively deeper waters as they mature and grow, and adults have been caught as deep as 2500 m, living on or in just above the southern shelves and slopes around the sub-Antarctic islands of the Southern Ocean. As apex predators, they are a key part of the food web, feeding on a variety of prey, including krill, squid, and other fish. Despite its importance, genomic sequence data, which could be used for more accurate dating of the divergence between Patagonian and Antarctic toothfish, or establish whether it shares adaptations to temperature with fish living in more polar or equatorial climes, has so far been limited. RESULTS: A high-quality D. eleginoides genome was generated using a combination of Illumina, PacBio and Omni-C sequencing technologies. To aid the genome annotation, the transcriptome derived from a variety of toothfish tissues was also generated using both short and long read sequencing methods. The final genome assembly was 797.8 Mb with a N50 scaffold length of 3.5 Mb. Approximately 31.7% of the genome consisted of repetitive elements. A total of 35,543 putative protein-coding regions were identified, of which 50% have been functionally annotated. Transcriptomics analysis showed that approximately 64% of the predicted genes (22,617 genes) were found to be expressed in the tissues sampled. Comparative genomics analysis revealed that the anti-freeze glycoprotein (AFGP) locus of D. eleginoides does not contain any AFGP proteins compared to the same locus in the Antarctic toothfish (Dissostichus mawsoni). This is in agreement with previously published results looking at hybridization signals and confirms that Patagonian toothfish do not possess AFGP coding sequences in their genome. CONCLUSIONS: We have assembled and annotated the Patagonian toothfish genome, which will provide a valuable genetic resource for ecological and evolutionary studies on this and other closely related species.


Assuntos
Perciformes , Animais , Perciformes/genética , Genômica , Regiões Antárticas , Evolução Biológica , Proteínas Anticongelantes
3.
Artigo em Inglês | MEDLINE | ID: mdl-38329409

RESUMO

BACKGROUND: People with behavioural variant frontotemporal dementia, Lewy body dementia, posterior cortical atrophy and young onset Alzheimer's disease may experience language and communication difficulties. However, the role of speech and language interventions for people with these non-language led dementias has received little attention. AIMS: This study aimed to explore the experiences and perspectives of people living with these conditions, and their families, regarding their language and communication difficulties and how speech and language therapy could address these needs. METHODS: This study employed a qualitative design to explore the experiences of people living with or caring for somebody with behavioural variant frontotemporal dementia, Lewy body dementia, posterior cortical atrophy or young onset Alzheimer's disease, and to understand their opinions about speech and language therapy. Participants were recruited from a support service connected to a dementia clinic to attend one of five focus group meetings. Videorecorded focus groups and interviews were transcribed, and reflexive thematic analysis was used to analyse data from people affected by each type of dementia. RESULTS: A total of 25 participants were recruited to the study, with representation across the different forms of non-language led dementias. The four main themes identified were: (1) communication difficulties as a key difficulty, (2) loss and loneliness, (3) speech and language therapy, and (4) the role of the caregiver. Sixteen subthemes were also identified which highlighted individual issues across disease types. DISCUSSION: Although all the forms of dementia studied here are not considered to be language-led, people with these conditions and/or their care partners identified speech, language and communication as common challenges. These communication difficulties were reported to have a negative impact on their social participation and mental health and participants felt speech and language interventions could help. There is a need for research exploring speech and language interventions developed for and with people with non-language led dementias and their care partners, to ensure they meet the needs of the people they are designed for. WHAT THIS PAPER ADDS: What is already known on the subject People with primary progressive aphasia present with speech, language and communication difficulties, and several speech and language interventions have been developed to meet the needs of this population. However, people with non-language led dementias may also experience speech, language and communication difficulties, and little is known about interventions that may address these difficulties. What this paper adds to existing knowledge People living with or caring for somebody with behavioural variant frontotemporal dementia, Lewy body dementia, posterior cortical atrophy and young onset Alzheimer's disease report experiencing speech, language and communication difficulties that impact on the person with dementia's social participation and mood. Participants in this study also shared their opinions about how speech and language interventions could help, from the earliest stages of the disease. What are the potential or actual clinical implications of this work? Speech and language therapists need to address the individual speech, language and communication needs of people with dementias, even those that are not thought to be language-led. Current speech and language therapy service provision does not meet the needs of people with non-language led dementias and further research is required to develop interventions and services to meet these needs.

4.
PLoS One ; 18(11): e0294458, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37971996

RESUMO

BACKGROUND: Evaluating community-based programs provides value to researchers, funding entities, and community stakeholders involved in program implementation, and can increase program impact and sustainability. To understand factors related to program implementation, we aimed to capture the perspective of community partners engaged in organizing and executing community-engaged programs to distribute COVID-19 at-home tests in underserved communities. METHODS: We conducted semi-structured interviews and focus groups with community-based stakeholders informed by the Outcomes for Implementation Research framework. RESULTS: Findings describe how community-engaged communication and dissemination strategies drove program adoption among grassroots stakeholders. Establishing and sustaining trusted relationships was vital to engaging partners with aligned values and capacity. Respondents characterized the programs as generally feasible and appropriate, and community partners felt capable of delivering the program successfully. However, they also described an increased burden on their workforce and desired more significant support. Respondents recognized the programs' community engagement practices as a critical facilitator of acceptability and impact. DISCUSSION: Implementation evaluation aims to inform current and future community outreach and engagement efforts with best practices. As we continue to inform and advance community-engaged disaster response practice, a parallel reimagining of public health funding mechanisms and timelines could provide a foundation for trust, collaboration, and community resiliency that endures beyond a given crisis.


Assuntos
COVID-19 , SARS-CoV-2 , Humanos , Autoteste , Populações Vulneráveis , COVID-19/diagnóstico , COVID-19/epidemiologia , Serviços de Saúde Comunitária , Teste para COVID-19
5.
Artigo em Inglês | MEDLINE | ID: mdl-37848225

RESUMO

Background: Reliance on informal caregivers is expected to increase, yet there are not many studies examining their characteristics and behaviors. Purpose: The aim of this review is to relate the interaction model of client health behavior (IMCHB) to informal caregiver outcomes. Methods: The literature associated with the IMCHB was examined for current usage to determine use in guiding theories related to informal caregiver outcomes. Cumulative Index to Nursing and Allied Health Literature, Cochrane, EBSCO, Google Scholar, Journals@Ovid, Medical Literature Analysis and Retrieval System Online, and ScienceDirect databases were searched for peer-reviewed literature. Walker and Avant's method guided this theoretical analysis. Results: Seven articles were retained for discussion in this theoretical analysis. Implications: The IMCHB has been used in past health promotion research and can guide future nursing research that supports interventions for informal caregivers. The model's inclusion of the client-professional interaction component allows for further caregiver behavioral associations and relationships that influence optimal outcomes.

6.
J Palliat Med ; 26(9): 1295-1296, 2023 09.
Artigo em Inglês | MEDLINE | ID: mdl-37672246
7.
J Adv Nurs ; 79(10): 3737-3747, 2023 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-37530425

RESUMO

BACKGROUND: With healthcare undergoing rapid digitalization, the effective integration of new technologies is crucial for nursing professionals, who form the largest group in the healthcare workforce. However, barriers within the nursing profession may impede digitalization efforts, leading to under utilization of available technologies and missed opportunities for enhancing healthcare quality and population health. AIMS: This article aims to investigate the adoption and use of digital technologies by nurses, considering how key demographics, such as gender, age, and voluntariness of technology use, interact to influence their acceptance and utilization of these technologies. METHODS: Employing the Unified Theory of Acceptance and Use of Technology (UTAUT) as a framework, we conducted a discursive exploration, supplemented by a literature review from diverse academic sources. Keywords related to UTAUT, digitalization, nursing practice and technology adoption were searched on PubMed, CINAHL and Google Scholar. Additionally, UK government and professional regulator reports were examined to understand current recommendations concerning digital technologies in nursing practice and the profession's demography. Searches focused on moderating factor domains, and the last search was conducted on 26 April 2023. RESULTS: The study revealed that the successful implementation of digital technologies in nursing practice requires a nuanced understanding of the nursing workforce's characteristics and preferences. Gender, age and voluntariness of technology use were found to intersect and influence nurses' acceptance and utilization of digital tools. DISCUSSION: By applying UTAUT in the context of nursing, this study highlights the importance of tailored implementation strategies for digital technologies. A technologically deterministic perspective is insufficient; instead, consideration of social factors specific to nursing is essential for successful adoption. CONCLUSION: To maximize the benefits of digitalization in healthcare, it is imperative to address the barriers faced by nursing professionals. A comprehensive understanding of how key demographics impact technology adoption will inform targeted strategies, enhancing the engagement of nurses with digital tools and fostering innovation in healthcare practices. Further research and primary data are needed, but this study lays the foundation for future advancements in digital healthcare integration for nursing professionals. RELEVANCE TO CLINICAL PRACTICE: The issues highlighted in this article are relevant to nurse leaders and those responsible for implementing technologies within nursing contexts. They are also relevant to technology developers who may benefit from considering the evidence associated with the moderating demographic factors highlighted in this article. Without a holistic approach to the implementation of technology, challenges associated with the use of digital technology by nurses are likely to persist. By considering the moderating demographic factors highlighted within the UTAUT (age, gender, voluntariness of use and experience) nurse leaders and technology developers may have greater success obtaining greater clinical outcomes from digital technology. This work was completed in 2022. NO PATIENT OR PUBLIC CONTRIBUTION: Due to the focus of this article being one on professional challenges within the nursing profession, no involvement from patients or the public was sought.


Assuntos
Tecnologia Digital , Enfermeiras e Enfermeiros , Humanos , Atenção à Saúde , Pessoal de Saúde , Qualidade da Assistência à Saúde
8.
Res Theory Nurs Pract ; 37(2): 195-213, 2023 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-37263637

RESUMO

Background: Compassion fatigue is a negative consequence impacting caregivers of chronic illness, such as heart failure. Reliance on family caregivers is expected to rise, placing importance on recognizing family caregiver characteristics that contribute to compassion fatigue. Purpose: This study specifically examined the associations of characteristics of family caregivers caring for end-stage heart failure, compassion satisfaction, social desirability, and the family caregiver-nursing provider relationship on compassion fatigue. Methods: An adapted interaction model of client health behavior guided this cross-sectional survey, which comprised a demographic questionnaire, the Professional Quality-of-Life Scale, the caregiver-provider relationship assessment, the Marlowe-Crowne Social Desirability Scale Short Form (Form C), and the Bakas Caregiving Outcomes Scale. Results: There were 127 family caregivers (FCGs), primarily those who have been a caregiver for longer than 1 year (78.4%), who completed the entire survey. Increased compassion satisfaction and positive caregiving effects were associated with decreased compassion fatigue (p < .001), while increased social desirability was associated with increased compassion fatigue (p < .001). A positive family caregiver-nursing provider relationship was related to decreased compassion fatigue (p < .001). More compassion fatigue was seen with lower spiritual status (p < .001). Implications: The chronic progression of heart failure presents opportunities for nursing providers to assess caregivers and offer frequent interventions. Family caregivers may not ask for help. Nursing providers must foster relationships with family caregivers to prevent negative consequences and mitigate compassion fatigue. Future research on the nursing provider relationship and FCGs is needed.


Assuntos
Cuidadores , Fadiga por Compaixão , Humanos , Estudos Transversais , Empatia , Doença Crônica , Inquéritos e Questionários , Qualidade de Vida
9.
Br J Nurs ; 32(9): 442-446, 2023 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-37173087

RESUMO

Digital technology is becoming increasingly common in routine nursing practice. The adoption of digital technologies such as video calling, and other digital communication, has been hastened by the recent COVID-19 pandemic. Use of these technologies has the potential to revolutionise nursing practice, leading to potentially more accurate patient assessment, monitoring processes and improved safety in clinical areas. This article outlines key concepts related to the digitalisation of health care and the implications for nursing practice. The aim of this article is to encourage nurses to consider the implications, opportunities and challenges associated with the move towards digitalisation and advances in technology. Specifically, this means understanding key digital developments and innovations associated with healthcare provision and appreciating the implications of digitalisation for the future of nursing practice.


Assuntos
COVID-19 , Humanos , Pandemias , Atenção à Saúde , Comunicação
10.
Child Abuse Negl ; 140: 106158, 2023 06.
Artigo em Inglês | MEDLINE | ID: mdl-36996592

RESUMO

BACKGROUND: Recent ACE research proposed items to assess ACE dimensions, such as the frequency or timing of adverse events, that can be added to the original ACE-Study Questionnaire. OBJECTIVE: The goal of our study was to pilot-test the refined ACE-Dimensions Questionnaire (ACE-DQ) to determine its predictive validity and compare scoring approaches. PARTICIPANTS AND SETTING: Cross-sectional online survey via MTurk with U.S. adults to collect data on the ACE-Study Questionnaire and the newly developed ACE dimension items, and mental health outcomes. METHODS: We compared ACE exposure by assessment approach and their associations with depression outcomes. We used logistic regression to compare the predictive validity of different ACE scoring approaches for depression outcomes. RESULTS: Participants (n = 450) were on average 36 years old, half were female, and the majority was White. Almost half reported depressive symptoms; approximately two-thirds had experienced ACEs. Participants reporting depression had significantly higher ACE scores. Using the ACE index, participants with ACEs were 45 % more likely to report depression symptoms than participants without ACEs (OR 1.45, 95%CI 1.33-1.58). When using perception-weighted scores, participants had smaller, yet significant odds of reporting depression outcomes. CONCLUSIONS: Our results suggest that the ACE index may overestimate the impact of ACEs and the effects of ACEs on depression. Adding the comprehensive set of conceptual dimensions to more fully weigh participants' experience of adverse events can increase the accuracy of ACE measurement but will also increase participant burden considerably. We recommend including items to assess a person's perception of each adverse event for improved screening efforts and in research focused on cumulative adversity.


Assuntos
Experiências Adversas da Infância , Depressão , Adulto , Humanos , Masculino , Feminino , Depressão/epidemiologia , Depressão/psicologia , Projetos Piloto , Estudos Transversais , Inquéritos e Questionários
11.
J Adv Nurs ; 79(11): 4137-4148, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36971282

RESUMO

AIMS: This scoping review aims to identify existing theories associated with digital nursing practice to add a lens on the future use of digital technologies by nurses. DESIGN: A review of theories related to digital technology in nursing practice was conducted following the framework described by Arksey and O'Malley. All published literature until 12th May 2022 was included. DATA SOURCES: Seven databases were utilized including Medline, Scopus, CINAHL, ACM Digital Library, IEEE Xplore, BNI and Web of Science. A Google Scholar search was also performed. REVIEW METHODS: The search terms included (nurs* AND [digital OR technol* OR e-health or ehealth or digital health or telemedicine or telehealth] AND theory). RESULTS: The database searches yielded 282 citations. After screening, nine articles were included in the review. These described eight distinct nursing theories. CONCLUSION: The focuses of the theories included the role of technology in society and nursing. How technology should be developed to support nursing practice, health consumers' use of nursing informatics, the use of technology as an expression of caring and the preservation of humanness and the relationship between human persons and non-human actants and the creation of nursing technologies as caring in addition to existing technologies. Three themes were identified including the role of technology as an agent within the patient environment; nurse interactions with technology to achieve 'knowing' of patients and the necessity of technological competence among nurses. Then, using Actor Network Theory (ANT), a zoom-out lens to map the concepts was proposed (The Lens for Digital Nursing [LDN]). This study is the first to add a new theoretical lens on digital nursing. IMPACT: This study provides the first synthesis of key concepts of nursing theories to add a theoretical lens to digital nursing practice. This can be used in a functional capacity to zoom-in different entities. No patient or public contribution was made in this study due to it being an early scoping study on a currently understudied area of nursing theory.


Assuntos
Informática em Enfermagem , Telemedicina , Humanos , Tecnologia
12.
J Hosp Palliat Nurs ; 24(5): 249-257, 2022 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-35881680

RESUMO

Before the COVID-19 pandemic, family caregivers were providing a tremendous amount of care for family members with heart failure with the prevalence of caregiver reliance in heart failure expected to increase in the United States. Social distancing and other restrictions during the COVID-19 pandemic may have added further challenges to caregiving routines. The purpose of this study was to examine the family caregiver perception of the effect of COVID-19 on caregiving routines. To determine caregiver perception of COVID-19's impact, 1 Likert question and 1 open-ended response were asked. Braun and Clark's method guided open-ended response thematic analysis. The 113 replies to the open-response question yielded themes such as social isolation, added fear, anxiety, or worry, changed appointments, wearing masks, and living status change. Social isolation (41.6%) was the most common theme; the most significant theme was living status change ( P = .003), and family caregivers reported that the pandemic affected their routines either negatively or somewhat negatively (62.1%). Family caregivers are affected during times of crisis. Research and policies that recognize the residual effects of COVID-19 on caregiving practices and support care transitions for family caregivers in the heart failure population are needed.


Assuntos
COVID-19 , Insuficiência Cardíaca , Cuidadores , Família , Insuficiência Cardíaca/complicações , Humanos , Pandemias
13.
J Palliat Med ; 25(5): 836-837, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-35499370
15.
J Hosp Palliat Nurs ; 22(5): 363-376, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32740303

RESUMO

The purpose of this integrative review was to appraise the qualitative and quantitative literature for community-level health behavior theory application to palliative care nursing to identify their use and future research implications. To date, there has not been an integrative review of the nursing studies that have used community-level health behavior theory to guide palliative care nursing research. Despite the availability of high-quality care, there continues to be underuse of resources. An integrative review on community-level theory application may provide a more holistic understanding of previous interventions to frame future interventions and research needs. The review was guided by the 5-step framework of Whittemore and Knafl. Relevant literature was searched and appraised. Seven different community-level health behavior theories were found with applications to palliative care nursing, and 16 studies using these theories were identified for discussion. Community-Based Participatory Research was the most used theory. Community-level health behavior theories such as Community-Based Participatory Research have helped build partnerships and activate community resources such as capacity, engagement, and diversity through culturally sensitive training interventions. Further research using these theories in palliative care nursing can realize positive outcomes, particularly in low-income rural areas.


Assuntos
Serviços de Saúde Comunitária/métodos , Cuidados Paliativos/métodos , Serviços de Saúde Comunitária/normas , Serviços de Saúde Comunitária/estatística & dados numéricos , Humanos , Cuidados Paliativos/normas , Cuidados Paliativos/estatística & dados numéricos
16.
Food Environ Virol ; 11(3): 229-237, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31165999

RESUMO

Human noroviruses are the leading cause of viral gastroenteritis. In the absence of a practical culture technique for routine analysis of infectious noroviruses, several methods have been developed to discriminate between infectious and non-infectious viruses by removing non-viable viruses prior to analysis by RT-qPCR. In this study, two such methods (RNase and porcine gastric mucin) which were designed to remove viruses with compromised capsids (and therefore assumed to be non-viable), were assessed for their ability to quantify viable F-specific RNA bacteriophage (FRNAP) and human norovirus following inactivation by UV-C or heat. It was found that while both methods could remove a proportion of non-viable viruses, a large proportion of non-viable virus remained to be detected by RT-qPCR, leading to overestimations of the viable population. A model was then developed to determine the proportion of RT-qPCR detectable RNA from non-viable viruses that must be removed by such methods to reduce overestimation to acceptable levels. In most cases, nearly all non-viable virus must be removed to reduce the log overestimation of viability to within levels that might be considered acceptable (e.g. below 0.5 log10). This model could be applied when developing alternative pre-treatment methods to determine how well they should perform to be comparable to established infectivity assays.


Assuntos
Capsídeo/efeitos da radiação , Norovirus/química , Norovirus/efeitos da radiação , Inativação de Vírus/efeitos da radiação , Animais , Biocatálise , Infecções por Caliciviridae , Capsídeo/metabolismo , Infecções por Enterovirus/virologia , Mucinas Gástricas/farmacologia , Temperatura Alta , Humanos , Norovirus/genética , Norovirus/fisiologia , Reação em Cadeia da Polimerase em Tempo Real , Ribonucleases/química , Suínos , Raios Ultravioleta
17.
J Hosp Palliat Nurs ; 21(1): 21-28, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30608916

RESUMO

The purpose of this review was to define compassion fatigue in the context of palliative care nursing. Compassion fatigue was first introduced as a description for nursing burnout; however, it was not fully described. An initial concept analysis within nursing placed it in terms of a psychological model for secondary traumatic stress disorder, with continual revisions of this application. Palliative care nurses are routinely exposed to pain, trauma, and the suffering they witness by nature of ongoing symptom management and end-of-life care delivery; however, the focus of care is on healthy end-of-life management rather than preservation of life. The literature was reviewed to provide clarification of compassion fatigue for palliative care nurses to assist in future identification and direction in the profession. CINAHL, EBSCO, Journals@Ovid, MEDLINE, PsycINFO, PubMed, and ScienceDirect databases were queried for peer-reviewed literature, and dictionaries were examined for subject-specific definitions. The method that was used was a concept analysis in the tradition of Walker and Avant. A concept definition was proposed for the discipline of palliative care nursing. Identification of compassion fatigue for this profession helps facilitate the recognition of symptoms for a group that deals with patient suffering on a regular basis.


Assuntos
Fadiga por Compaixão/etiologia , Enfermagem de Cuidados Paliativos na Terminalidade da Vida/normas , Fadiga por Compaixão/enfermagem , Fadiga por Compaixão/psicologia , Enfermagem de Cuidados Paliativos na Terminalidade da Vida/métodos , Humanos , Satisfação no Emprego , Estresse Psicológico/complicações , Estresse Psicológico/psicologia
18.
Int J Bacteriol ; 2015: 593745, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26904747

RESUMO

We report a novel approach utilising a real-time PCR screening assay targeting a 53 bp tandemly repeated element present at various loci within the Mycobacterium tuberculosis (Mtb) genome. Positive samples were identified within a discriminatory melting curve range of 90-94°C, with results obtained in under one hour directly from decontaminated sputum samples without extraction. A panel of 89 smear-positive sputa were used for analytical validation of the assay with 100% concordance, with sensitivity matching that of culture. Cross reactivity was detected within a narrow range of mycobacteria other than tuberculosis (MOTT) (five sputa, three in silico), with the highest sensitivity within M. avium complex (MAC). A year-long head to head evaluation of the test with the GeneXpert platform was carried out with 104 consecutive samples at the Royal Free Hospital, UK. Receiver operating characteristics (ROC) analysis of the data revealed that the two tests are approximately equivalent in sensitivity, with the area under the curve being 0.85 and 0.80 for the GeneXpert and our assay, respectively, indicating that the test would be a cost effective screen prior to GeneXpert testing.

19.
Emerg Infect Dis ; 19(8): 1302-4, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23877005

RESUMO

Shiga toxin-producing Escherichia coli serotype O117:K1:H7 is a cause of persistent diarrhea in travelers to tropical locations. Whole genome sequencing identified genetic mechanisms involved in the pathoadaptive phenotype. Sequencing also identified toxin and putative adherence genes flanked by sequences indicating horizontal gene transfer from Shigella dysenteriae and Salmonella spp., respectively.


Assuntos
Genoma Bacteriano , Escherichia coli Shiga Toxigênica/genética , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Funções Verossimilhança , Tipagem de Sequências Multilocus , Filogenia , Polimorfismo de Nucleotídeo Único , Sorotipagem , Escherichia coli Shiga Toxigênica/classificação
20.
FEMS Microbiol Lett ; 321(2): 100-6, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21623896

RESUMO

Fluorescent amplified fragment length polymorphism (FAFLP) analysis was applied to genetically fingerprint 'working culture control strains' used by accredited food microbiology laboratories. A working culture control strain is defined as a subculture from a strain initially obtained from an authenticated source [such as the National Collection of Type Cultures (NCTC)] that is maintained for use with routine testing within the laboratory. Working culture control strains from eight food examination laboratories, representing four bacterial species, were analysed by FAFLP; these were Salmonella Nottingham, Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus. The resultant FAFLP profiles of the eight working culture control strains for each of these species were compared against the appropriate freeze-dried ampoules obtained directly from NCTC. FAFLP results demonstrated that within 50% of working cultures analysed, several laboratories were routinely using working cultures that were genetically different from the original reference NCTC strains. This study highlights the need for laboratories to review the protocols used to process and maintain control strains and working cultures, with a potential view to utilize single-use quality control materials.


Assuntos
Análise do Polimorfismo de Comprimento de Fragmentos Amplificados/normas , Microbiologia Ambiental/normas , Microbiologia de Alimentos/normas , Tipagem Molecular/normas , Bacillus cereus/genética , Variação Genética , Listeria monocytogenes/genética , Padrões de Referência , Reprodutibilidade dos Testes , Salmonella/genética , Staphylococcus aureus/genética
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